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301 302 301 330023 304 330035 330046 307 305 306 aureus; P.A.-P. aeruginosa; E.F.-E. faecalis; K.A.-K. pneumoniae 308 various concentrations (100 to 12.5 μg/ml) of AgNPs synthesized from Nigella sativa extract. E.C.- E. coli; S.A.- S. 307 3.7. Effect of Ns-AgNPs Against Human (HCC712) Cell Lines 309 The cytotoxic effect of Ns-AgNPs in the current study was examined against HCC712 (human Processes 2019, 7, x FOR PEER REVIEW Processes 2020, 8, 388 Processes 2019, 7, x FOR PEER REVIEW Figure 6. Image showing the screening of biofilm inhibition in the presence of Ns-AgNPs. Figure 6. Image showing the screening of biofilm inhibition in the presence of Ns-AgNPs. Row Row A & FBi-gNuergea6t.ivIemcaognetrsohlo(Nw.iCn.g): tChueltsucreemneindgiaofnblyio, fNilombianchteibriatilocnolionntyhaenpdrensoenNcse-Aogf NPss-A; RgoNwPsC. & D- A&B-Negative control (N.C.): Culture media only, No bacterial colony and no Ns-AgNPs; Row Positive control (P.C.): Culture media plus 5 different bacterial colonies in different wells accordingly and no C&D-Positive control (P.C.): Culture media plus 5 different bacterial colonies in different wells R N o s w - A A g N & P s B ; - R N o e w g a E t i t v o e H c o - n C t r u o l t l u ( r N e . mC . e ) : d C i a u p l t l u u r s e 5 m d e i f d f e i a r e o n n t l b y a , c N t e o r i b a a l c c t o e l r o i n a i l e c s o i l n o n d y i f f a e n r d e n n t o w N e l s l s - A a g c c N o P r d s i ; n R g o l y w a C n d & D - accordingly and no Ns-AgNPs; Row E to H-Culture media plus 5 different bacterial colonies in different Pvoasritoiuvseccoonncternotlra(tPio.Cns.):(1C0u0lttou1re2.5mμegd/imalp)loufsA5gdNiPffsesryenthbeasciztedriafrlocmoloNnigielslainsadtivifaferxetnratcwt.eEl.lCs.a-cEc.ocrodli;nSg.Aly.-aSn.d no wells accordingly and various concentrations (100 to 12.5 μg/mL) of AgNPs synthesized from Nigella Ns-AgNPs; Row E to H- Culture media plus 5 different bacterial colonies in different wells accordingly and sativa extract. E.C.-E. coli; S.A.-S. aureus; P.A.-P. aeruginosa; E.F.-E. faecalis; K.A.-K. pneumoniae 3.7. Effect of Ns-AgNPs Against Human (HCC712) Cell Lines aureus; P.A.-P. aeruginosa; E.F.-E. faecalis; K.A.-K. pneumoniae 10 of 14 10 of 14 10 of 14 308 The cytotoxic effect of Ns-AgNPs in the current study was examined against HCC712 (human 310 311 3.7. Effect of Ns-AgNPs Against Human (HCC712) Cell Lines 309 breast cancer cells) using MTT assay. The toxicities were examined at different concentrations (25 to 200 μg/ml) and compared to control. The percent inhibition of cell growth was increased with the 312 200μTgh/me cLy)taontodxiccomeffpeacrteodftNosc-oAngtNroPl.sTinhethpeecrcuernretninthstiubidtyiownaosfceexlalmgrinoewdthagwaainssitnHcrCeaCs7e1d2w(hituhmtahne concentration of the nanoparticles (Figure 7). The inhibition effect was time and dose dependent and 310 cborenacsetnctraantcioerncoeflltsh)eunsaingopMaTrtTicalessa(Fyi.gTuhre t7o).xiTchiteieisnwhiebrietioenxaemffiencetdwatsdtimfferaendt cdoonscendterpaetinodnesn(t2a5ntdo the IC50 value of 150 μg/ml supports the levels of the inhibition. 313 311 312 313 t2h0e0 IμCg/mvla)luaendofc1o5m0 pμagr/emdLtosucpopnotrotsl.tThehelepverlsceonftthinehiinbhitiibointionf. cell growth was increased with the 50 314 Figure 7. MTT assay results confirming the in-vitro cytotoxicity of the Ns-AgNPsagainst HCC 712 human breast cancer cell-lines. Figure 7. MTT assay results confirming the in-vitro cytotoxicity of the Ns-AgNPsagainst It was observed that a high concentration of Ns-AgNPs showed promising activity against breast 315 316 HCC 712 human breast cancer cell-lines. 314 cancer cell lines and cell proliferation decreased after 24 h. Moreover, findings exhibited that 150 μg/mL 315 316 concentration of AgNPs is able to provoke the approximately 50% of cell mortality at different time Figure 7. MTT assay results confirming the in-vitro cytotoxicity of the Ns-AgNPsagainst breast cancer cells) using MTT assay. The toxicities were examined at different concentrations (25 to concentration of the nanoparticles (Figure 7). The inhibition effect was time and dose dependent and the IC50 value of 150 μg/ml supports the levels of the inhibition. intervals. The cell viability in the presence of AgNPs at different concentrations is illustrated in HCC 712 human breast cancer cell-lines. (Figure 8 A–F). The calculated IC50 value is approximately 175 μg/mL concentrations of Ns-AgNPs.PDF Image | Silver Nanoparticles Seed Extract Nigella sativa blackseed
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