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Processes 2020, 8, 388 12 of 14 S. aureus, and E. faecalis. The current study findings revealed that Ns-AgNPs showed a significant cytotoxic effect on HCC712 breast cancer cell lines. In summary, our results a suggest cost-effective and eco-friendly approach for Ns-AgNps synthesis which may support the advancement of potential biomedical applications and alternative therapy involving the design of novel antibiofilm agents and multi drug resistance bacterial infection, and further could be developed as a template for other anti-cancer actions. Author Contributions: A.A. design the conceptualized research study. H.K. and M.A. executed the detailed analysis and drafted manuscript F.K.A.K. and M.A.A. drafted manuscript. K.S.A. and A.H.R. given potential input in results interpretation. R.K. supervised the whole process. All authors have read and agreed to the published version of the manuscript. Funding: This research work was financially supported by Deanship of scientific research grant, Qassim University, KSA. Grant number: 3970-cams1-2018-1-14-S. Acknowledgments: Authors like to thanks to their respective institutions for sophisticated analytical instrumentation facilities. The authors acknowledge deanship of scientific research (DSR/grant number/year) Qassim University, kingdom of Saudi Arabia, providing finance support for research project fund. Conflicts of Interest: The authors declare no conflict of interest. Appendix A References Ns AgNPs DLS UV-Vis FTIR SEM EDX Nigella sativa Silver nanoparticles Dynamic light scattering Ultraviolet visible Fourier-Transform Infrared Radiometer Scanning light microscope Energy dispersed X-ray 1. Naganathan, K.; Thirunavukkarasu, S. Green way genesis of silver nanoparticles using multiple fruit peels waste and its antimicrobial, anti-oxidant and anti-tumor cell line studies. In Conference Series: Materials Science and Engineering; IOP Publishing: Bristol, UK, 2017. 2. Choi, S.M.; Seo, M.H.; Kim, H.J.; Kim, W.B. Synthesis and characterization of graphene-supported metal nanoparticles by impregnation method with heat treatment in H2 atmosphere. Synth. Met. 2011, 161, 2405–2411. [CrossRef] 3. Das, G.; Patra, J.K.; Debnath, T.; Ansari, A.; Shin, H.-S. Investigation of antioxidant, antibacterial, antidiabetic, and cytotoxicity potential of silver nanoparticles synthesized using the outer peel extract of Ananas comosus (L.). PLoS ONE 2019, 14, e0220950. [CrossRef] [PubMed] 4. Erdogan, O.; Abbak, M.; Demirbolat, G.M.; Birtekocak, F.; Aksel, M.; Pasa, S.; Ozge, C. Green synthesis of silver nanoparticles via Cynara scolymus leaf extracts: The characterization, anticancer potential with photodynamic therapy in MCF7 cells. PLoS ONE 2019, 14, e0216496. [CrossRef] [PubMed] 5. Hamouda, R.A.; Hussein, M.H.; Abo-elmagd, R.A.; Bawazir, S.S. Synthesis and biological characterization of silver nanoparticles derived from the cyanobacterium Oscillatoria limnetica. Sci. Rep. 2019, 9, 13071. [CrossRef] 6. Afifa, Q.; Rajesh, K.; Anupam, D. Green synthesis of silver nanoparticles by seed of Phoenix sylvestris L. and their role in the management of cosmetics embarrassment. Green Chem. Lett. Rev. 2018, 11, 176–188. 7. Nayak, D.; Ashe, S.; Rauta, P.R.; Kumari, M.; Nayak, B. Bark extract mediated green synthesis of silver nanoparticles: Evaluation of antimicrobial activity and antiproliferative response against osteosarcoma. Mater. Sci. Eng. C 2016, 58, 44–52. [CrossRef] 8. Gao, X.; Yourick, J.J.; Topping, V.D.; Black, T.; Olejnik, N.; Keltner, Z. Toxicogenomic study in rat thymus of F1 generation offspring following maternal exposure to silver ion. Toxicol. Rep. 2015, 2, 341–350. [CrossRef] 9. Mah, T.F.C.; O’Toole, G.A. Mechanisms of biofilm resistance to antimicrobial agents. Trends Microbiol. 2001, 9, 34–39. [CrossRef]PDF Image | Silver Nanoparticles Seed Extract Nigella sativa blackseed
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