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Nanomaterials 2020, 10, 2255 6 of 11 The aged BPEI AgNPs also showed much higher toxicity than non-aged BPEI AgNPs (Figure 4). The EC50 of PEG AgNPs and BPEI AgNPs to Hepa1c1c7 cells decreased four and five times after one year of aging, respectively (Figure 4C). Figure 3. TEM images of surface-modified AgNPs. A0–D0: The initial images of Citrate AgNPs, PEG AgNPs, PVP AgNPs, and BPEI AgNPs. A1–D1: The one-year TEM images of Citrate AgNPs, PEG AgNPs, PVP AgNPs, and BPEI AgNPs in storage medium. Figure 4. Dynamics of AgNPs’ toxicity to Hepa1c1c7 cells. Data obtained from luminescent assay for silver nanoparticles in Hepa1c1c7 cells 24 h. (A). The toxicity of the pristine AgNPs to Hepa1c1c7 cells. (B) The toxicity of stored one-year AgNPs in lab to Hepa1c1c7 cells. The y-axis represents the percent ofPDF Image | Silver Nanoparticles Undergoing Long-Term Aging
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