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phenolic compounds and flavonoids. These compounds stabilized their structure and likely added novel properties to biosynthesized nanoparticles. 3.2.3. X-ray Diffraction (XRD) Crystalline properties of biosynthesized nanoparticles were evaluated using XRD (Figure 2B). Nanomaterials 2020, 10, 638 6 of 17 Main diffraction peaks identified in the diffractogram were at 2θ values of 27.9°, 32.4°, 46.2°, 54.8°, 57.6°, 67.5°, 74.3°, and 76.9o. They corresponded to (111), (200), (220), (311), (222), (400), (331), and in phenolic components and alcohols with strong hydrogen bonds [25]. All these FTIR absorption (420) planes, respectively, and confirmed the face-centered cubic (FCC) structure of AgCl according peaks verified that synthesized AgCl-NPs were capped with P. vulgaris metabolites, likely including to the database of Joint Committee on Powder Diffraction Standards (JCPDS), file number 31-1238. phenolic compounds and flavonoids. These compounds stabilized their structure and likely added XRD analysis confirmed that biogenic AgCl-NPs were synthesized [11]. According to the Debye– novel properties to biosynthesized nanoparticles. Sherrer equation, the average crystalline size of biosynthesized AgCl-NPs was 28.6 nm. Nanomaterials 2020, 10, x FOR PEER REVIEW 7 of 17 (A) (B) Figure 2. (A) The FTIR spectrum and (B) the XRD pattern of biosynthesized AgCl-NPs using aerial Figure 2. (A) The FTIR spectrum and (B) the XRD pattern of biosynthesized AgCl-NPs using aerial part extracts of the P. vulgaris plant. part extracts of the P. vulgaris plant. 3.2.4. Transmission Electron Microscopy (TEM) TEM analysis (Figure 3A and B) clearly confirmed that the shape of biosynthesized AgCl-NPs was spherical, while their size was ranged from 14.3 to 50.7 nm with the average of 28.6 ± 9.0 nm— that being the same as the particle size measured based on the XRD pattern.PDF Image | Synthesis of Biogenic Silver Nanoparticles Aerial Part Extract
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