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ZINC OXIDE AND SILVER NANOPARTICLES ON INTESTINAL BACTERIA

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ZINC OXIDE AND SILVER NANOPARTICLES ON INTESTINAL BACTERIA ( zinc-oxide-and-silver-nanoparticles-on-intestinal-bacteria )

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dynamics of bacterial growth was observed in liquid medium with 107 E. coli cells and 10, 50, and 100 μg cm-3 Ag NPs. All of the three different concentrations caused a growth delay of E. coli and increasing the concentration of NPs increased the growth delay. According to a study by Kim and others (2007), yeasts and E. coli were inhibited at low concentrations of Ag NPs. However, S. aureus was less inhibited by Ag NPs compared to yeast and E. coli O157:H7. To be more specific, the MIC of Ag NPs against yeast was between 6.6 nM and 13.2 nM and against E. coli O157:H7 was between 33 nM and 6.6 nM. As the concentrations of Ag NPs got higher, the stronger inhibitory effects were observed. MIC of Ag NPs against S. aureus was estimated to be more than 33 nM. The growth-inhibitory effect was mild against S. aureus even in high concentrations of Ag NPs compared to other microorganisms used in this study. A study by Raffi and others (2009) also showed antibacterial effects against E. coli 15224 in both liquid and solid growth media. The number of CFU was significantly reduced with increasing concentrations of Ag NPs. The concentrations of Ag NPs, as low as 60 μg/mL, showed complete cytoxicity to the E. coli bacterial strain. A study by Martinez-Castanon (2008) showed that smaller sizes of Ag NPs have larger surface to volume ratio which makes interaction between bacterial cells and NPs and the resulting antibacterial effects stronger. Three different sizes (7, 29, and 89 nm mean value) were synthesized by an aqueous chemical reduction method and the standard microdilution method was used to determine antibacterial activity of Ag NPs. As a result, 7 nm Ag NPs was most effective against both E. coli and S. aureus. For E. coli, there was no significant difference between the MIC of 29 and 89 nm Ag NPs. Also, the MIC of all 11

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