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with peptone water and plated on respective agar media, Tryptic Soy Agar supplemented with yeast extract for E. coli K-12 and Lactobacilli MRS agar supplemented with cysteine for L. acidophilus ADH and B. animails Bif-6, at 0, 6, 7, 8, 9, 10, 11, 12, 15, and 24 h. 3.4 Effect of Ag NPs on the growth of E. coli K-12, L. acidophilus ADH, and B. animalis Bif-6 All three strains, incubated overnight in respective broth media, TSBY and MRS, were inoculated into broth media containing different concentrations of Ag NP solution (0, 1.8, 2.7, 4.6 mM), and 1% of NP-free solution. For concentrations of Ag NPs, mg/mL unit was converted into mM unit. The NP-free solution was prepared by filtering the Ag NP solutions using an anodisc inorganic membrane (Whatman Inc.) with a pore size of 20 nm. Both Ag NP suspensions and the NP-free solution were added to the broth media before autoclaving. After inoculating the cultures into the TSB or MRS broth media containing Ag NP suspensions and NP-free solution, samples were incubated at 37 oC in a shaking incubator. The tubes inoculated with L. acidophilus ADH and B. animalis Bif-6 were placed in an anaerobic jar with GasPak (BD) and incubated in a shaking incubator. The samples were diluted with peptone water and plated on respective agar media, Tryptic Soy Agar supplemented with yeast extract for E. coli K-12 and Lactobacilli MRS agar supplemented with cysteine for L. acidophilus ADH and B. animails Bif-6, at 0, 1, 2, 3, 4, 5, 6, 9, 12, and 24 h. 20PDF Image | ZINC OXIDE AND SILVER NANOPARTICLES ON INTESTINAL BACTERIA
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