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4.3 Characterization of ZnO and Ag NPs The size and morphology of ZnO and Ag NPs were determined by TEM. The original ZnO NPs suspensions were diluted with ultra purified water and observed under TEM (Figure 4.3). Most of ZnO NPs were in either round or oval shape with an average size of 77.9 nm. The software ImageJ was used to analyze the sized of NPs. Figure 4.4 showed the histogram of the size distribution of ZnO NPs, and the majority of ZnO NPs measured fell in the range of 60 to 80 nm in diameter. ZnO NPs may be present in the form of agglomerates due to synthetic processing (Zhang and others, 2007). Therefore, ultrasonication and dispersants, such as polyethylene glycol (PEG), polyvinylpyrolidone (PVP) and bovine serum albumin (BSA) are often used to disintegrate NPs agglomerates (Brayner and others, 2006). However, as shown in Figure 4.3, ZnO NPs were relatively well dispersed with slight agglomeration in ultra-purified water without sonification or dispersant. Also, a shaking incubator was used to avoid the aggregation of ZnO NPs in the broth. 37PDF Image | ZINC OXIDE AND SILVER NANOPARTICLES ON INTESTINAL BACTERIA
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