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Energy dispersive spectroscopy (EDS) is a common technique for analysis of the elemental composition of a specimen. It is also capable of generating a map of multiple chemical elements of interest at specifically pointed spots. EDS coupled with SEM were used for elemental analysis and it was effective in locating and identifying NPs attached to the bacterial cells. Figure 4.13 shows SEM-EDS elemental analysis of ZnO NPs attached to E. coli cells. Control cells and ZnO NPs attached to cells were spotted to analyze the elements. Three spots were selected in each sample to analyze the elements. Results showed that no Zn elements were identified in the control cells, Figure 4.13A, which was as expected. Zn elements were observed in samples treated with ZnO NPs (Figure 4.13B and 4.13C) which indicated the presence of ZnO NPs. Also, relatively large amounts of platinum elements were observed in all samples. This is due to the thin layer of platinum coating to reduce the charges when observing under SEM. 47PDF Image | ZINC OXIDE AND SILVER NANOPARTICLES ON INTESTINAL BACTERIA
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