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4.4.2 Treated with Ag NPs As shown in Tables 4.3, 4.4, and 4.5, bacterial strains exposed to Ag NPs for 6 h showed the highest antimicrobial effect. Therefore, SEM and TEM images of E. coli, L. acidophilus, and B. animalis incubated in the respective broth medium for 6 h, with and without the presence of 4.6 mM of Ag NPs, were observed. Figures 4.14A and 4.14B showed SEM images of E. coli before and after treatment with Ag NPs. As shown in Figure 4.14A and 4.14B, no significant changes in the external appearance of the cells were observed after treatment with Ag NPs for 6 h. Ag NPs were attached to bacterial cells (Figure 4.14B) in clusters instead of a single particle, as shown in Figure 4.5. Similar agglomeration was observed in ZnO NPs too. Figure 4.15A showed intracellular structures of the E. coli control sample. The bacterial cells were normal in size with intact intracellular structures and well-organized intracellular contents. However, deformation of cell membrane and intracellular structure were observed in Figure 4.15B. Bacterial cells were surrounded by Ag NPs and null bacterial cells were found in bacterial sample treated with Ag NPs, indicating that intracellular contents had leaked from cells due to damage to the cell membrane. 49PDF Image | ZINC OXIDE AND SILVER NANOPARTICLES ON INTESTINAL BACTERIA
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