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4.5 Determination of membrane leakage To determine membrane leakage, a UV-visible spectrophotometer was used to monitor the absorbance at wavelength of 260 nm (DNA absorbance). The absorbance is related to the leakage amount of intracellular DNAs. Figure 4.21 shows the absorbance of bacterial strains after treatment of ZnO NPs for 10 h. For all three bacteria, the absorbance at 260 nm shows a slight increase after 10 h of exposure to ZnO NPs. Higher concentrations of ZnO NPs resulted in a higher absorbance. Figure 4.22 showed the absorbance of bacterial strains after treatment of Ag NPs for 6 h. The results were similar to the ZnO NPs results. Absorbance at 260 nm of all three bacteria increased slightly as compared to the control. The absorbance increased due to the leakage of nucleic acids from bacterial cells when their cell membranes are damaged. However, there were no significant differences (P ≤ .05) between the control and treated samples. 56PDF Image | ZINC OXIDE AND SILVER NANOPARTICLES ON INTESTINAL BACTERIA
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