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ethanol, and then diluted with ethanol as the working solutions. Copper standard solution (5.00×10−2 mol L−1) was prepared by dissolving Cu(CH3COO)2 x H2O in doubly distilled water, purchased from Merck. The working solutions of copper(II) were prepared by dilution of the standard solution with doubly distilled water. A stock solution of curcumin (5.00×10−3 M) was prepared just before use by dissolving CC in ethanol, purchased from Sigma. The supporting electrolytes were 50 mM sodium phosphate buffer solution + 0.3 M NaCl (pH 8.5) for measurement by hanging mercury drop electrode [32] and 0.2 M sodium acetate buffer solution + 20 mM NaCl (pH 5.0) for measurement by carbon paste electrode. For the study of the electrochemical behaviour of dsDNA on the CPE surface, the stock solutions (140 mg L-1) was prepared in 10 mM Tris-HCl and 1 mM EDTA at pH 8.0 [32]. 2.2 Apparatus Differential pulse voltammetric measurements was performed with a PalmSens potensiostat purchased from IVIUM Technologies (The Netherlands, www.ivium.nl) and PalmSensPC software. The working electrodes were a hanging mercury drop electrode (EA 290 Metrohm, www.metrohm.com, type 6.0335.000, surface area 2.22 mm2) and a carbon paste electrode with 3 mm inner and 9 mm outer diameter of the PTFE sleeve. The reference electrode was a saturated Ag / AgCl and the counter electrode was a platinum wire. 2.3 Preparation of working electrodes 2.3.1 Carbon paste electrode The carbon paste was prepared in the usual way by hand-mixing graphite powder and nujol oil to form homogeneous mixture. The ratio of graphite powder to nujol oil was 75:25. 4PDF Image | Electroanalytical dsDNA and curcumin vs copper
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