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Recently there have been many reports in the literature on the metal-chelating properties of curcumin, employing techniques like potentiometry and absorption spectroscopy [21-27]. Electrochemistry has been used with much success in metal–ligand studies [28,29]. Limson et al. [28] used electrochemistry to show metal–ligand interactions between the pineal hormone melatonin and a range of metals. Changes in current response and potential are good indicators of alterations in the chemistry of a target compound, such as that which occurs during an exchange of electrons in metal–ligand bond formation. Adsorptive stripping voltammetry exploits the natural tendency of analytes to pre-concentrate at an electrode and is a useful technique for investigation of metal–ligand interactions [30]. These considerations as well as all the other information about curcumin on the clinical and chemistry aspects have already been given in our previous paper [31] prompted us to electrochemicaly explore the interaction between calf-thymus DNA and curcumin in the presence of Cu(II). Our previous study [31] has explored the electrochemical properties of curcumin by cyclic and differential voltammetry. The present study is focused on the behavior of DNA exposed to curcumin and copper, based on the development of an electrochemical biosensor, a carbon paste electrode. As yet has been no literature report for the voltammetric detection of this interaction, by using differential pulse voltammetry. 2 Experimental 2.1 Reagents All the chemicals used were of analytical reagents grade. Double stranded (ds) calf thymus DNA (D-1501, highly polymerized) was purchased from Sigma. Graphite powder was purchased from Fluka (50870), p.a. purity (99,9%) and particle size < 0.1 mm. A stock solution of curcumin (5.00×10−3 mol L−1) was prepared just before use by dissolving CC in 3PDF Image | Electroanalytical dsDNA and curcumin vs copper
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