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The resulting paste was packed tightly into a PTFE sleeve and the electrode surface was polished to a smooth finish on a piece of weighing paper. Electrical contact was established with stainless steel screw. The constructed electrode was washed with distilled water and then was transferred to supporting electrolyte solution. The electrode was pre-treated by applying a potential at + 1.7 V for 1 min without stirring prior to the accumulation step. The electrochemical pre-treatment produces a more hydrophilic surface state and a concomitant removal of organic layers [33]. 2.3.2 Hanging mercury drop electrode The hanging mercury drop electrode (HMDE) was transferred to de-aerated supporting electrolyte solution. Ultra pure argon was used to bubble the solutions of dissolved oxygen. Initially the solution was bubbled with argon for 5 min. Additionally the blank background buffer was de-aerated for 1 min before each measurement. 2.4 Procedures 2.4.1 Differential pulse voltammetric study of the mixture of CC and Cu(II) solutions The study was performed in phosphate buffer pH 8.5, when HMDE was used. The conditions were Estep = 0.005 V, Epulse = 0.025 V and scan rate = 0.050 V s-1. In the case of CPE, acetate buffer pH 5.0 was used. The conditions were Estep = 0.005 V, Epulse = 0.025 V and scan rate = 0.050 V s-1. Stock CC and Cu(II) solutions were added to the proper buffer to produce the required concentrations and the mixture was left to stand to equilibrate for 10 min. 2.4.2 Treatment of dsDNA with CC and Cu(II) in solution and immobilization at the CPE surface 5PDF Image | Electroanalytical dsDNA and curcumin vs copper
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