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The analysis of solution-phase DNA with CC and Cu(II) consisted of mixing the three components, followed by accumulation and measurement of the peak current of guanine residues, by transfer voltammetry, using differential pulse as the stripping mode. The electrode was rinsed for 5 s prior to each medium exchange. Stock CC and Cu(II) solutions were added to 0.2 M acetate buffer to produce the required concentrations and the mixture was left to stand for 10 min. Thereafter, stock DNA (0.140 g L-1) solution was added to the mixture and a new mixture was left to stand for 5 min. A freshly polished carbon paste electrode, after the electrode pretreatment, was immersed into the solution. The accumulation of the mixture (DNA, CC and Cu(II)) was performed by applying a potential of +0.5 V for 5 min. The modified electrode was then removed from the solution and washed twice with doubly-distilled water and with the background electrolyte solution. The washed electrode was then placed into a voltammetric cell. The DP curve was recorded in the blank acetate buffer solution, from +0.1 V to positive/negative potential values direction. The conditions were Estep = 5 mV, Epulse = 25 mV and scan rate = 50 mV s-1. 3 Results and discussion 3.1 Curcumin–Cu(II) interaction In our previous paper [31] it has been studied the electrochemical properties of curcumin using voltammetric techniques and CPE. It has been shown that optimum deposition potential is 0.30 V and deposition time is 120 s for the reduction peak at 0.3 V. In the same condition, in this article, it has been conformed making complex between curcumin and Cu(II) [34], what is obvious from Figure 1. The presence of 2.00 x 10-5 M Cu(II), at pH 6PDF Image | Electroanalytical dsDNA and curcumin vs copper
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