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72 comfortable temperature and hum1d1ty For prec1se work a panel of five or more testers should be used The persons makmg the odor measurements should not prepare the samplesandshouldnotknowthedllutlOnconcentrat10nsbemgevaluated Thesepersons should have been made fam1har w1th the procedure before part1c1patmg m a panel test Always start,w1th the most dllute sample to aVOld tmng the senses w1th the concentrated sample The temperature of the samples dunng testmg should be kept w1thm 1 degree of the spec1fied temperature for the test Threshold measurement The ratio by wh1ch the odor-beanng sample has to be dlluted w1th odor-free water for the odor to be Just detectable by the odor test 1S the "threshold odor number" (T 0 N ) The total volume of sample and odor-free water used m each test 1S 200 ml The proper volume of odor-free water 1S put mto the flask first, the sample 1S then added to the water Table 1 g1ves the dllutlOns and correspondmg threshold numbers 7 3 Table 1 Threshold Odor Number Correspondmg to VarIOus Dilutions Sample Volume (ml) Dl1uted to 200 ml 200 100 Determme the approx1mate range of the threshold odor by 73 1 Addmg 200 ml, 50 ml, 125 ml, and 3 1 ml of the sample to separate 500 ml glass- 7 3 2 stoppered Erlenmeyer flasks contammg odor-free water to make a total volume of 200 ml A separate flask contammg only odor-free water serves as the reference for companson If run at 60~C, heat the dllutions and the reference m the constant temperature bath at 60°C (± 1°C) Shake the flask contammg the odor-free water, remove the stopper, and smff the vapors Test the sample contammg the least amount of odor-beanng water m the same way Ifodor can be detected m th1s dllutlOn, more dllute samples must be prepared as descnbed m (733) If odor cannot be detected m the first dllutlOn, repeat the above procedure usmg the sample contammg the next h1gher concentration of the odor-beanng wate1, and contmue tills process untll odor 1S clearly detected 2 50 4 25 8 125 16 63 32 31 64 16 128 08 256 140 1-3 Threshold Odor Number 1PDF Image | Methods for Chemical Analysis of Water and Wastes
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