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1CO2 UTILIZATION COOBAF The screening and identification of suitable bacterial strains that could ac- commodate the acetone operon and the introduction of the operon into some of the selected candidates was carried out in the group led by Profes- sor Bahl at Rostock University. The same group also attempted to integrate the acetone operon into the genome. The work packages completed by the Rostock group are listed below: Work package WP activities and tasks (Rostock University) AP 1.1: Cultivation of bacteria in a gas stream AP 1.2: Screening of bacterial strains AP 1.3: Cultivation of autotrophic strains AP 2: Heterologous autotrophic acetone formation AP 3.1 Integrating the acetone operon into the genome AP 3.2 Creating and analysing mutants The research group led by Professor Dürre from the University of Ulm focused on increasing acetone yield and productivity in the acetone- generating strains using physiological and genetic engineering techniques and on introducing the acetone operon into the remaining selected candidate strains. The work packages completed by the Ulm group are listed below: Work package WP activities and tasks (University of Ulm) AP 4: Optimisation of acetone synthesis AP 5: Transmission of the acetone synthesis gene AP 6: Sequencing of C. aceticum AP 7: Strain development At Evonik Industries AG project work focused on developing the fermentation process itself. This included characterising the most important parameters (e.g. pH, temperature) that influence the formation of the product and co-products and developing an appropriate fermentation model. In addition to developing the fermentation process at the lab scale, work was also un- dertaken to develop a cost- and yield-optimised acetone workup procedure. The work packages completed by at Evonik Industries AG are listed below: 64PDF Image | Chemical Processes and Use of CO2
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