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COOBAF FUNDED PROJECTS Work package WP activities and tasks (Evonik Industries AG) AP 8: Developing and establishing analytical methods; Setting up the experimental equipment AP 9: Development of the fermentation process AP 10: Downstream process development 1.7.3 Results Rostock University Both heterotrophic and autotrophic cultivation experiments were performed on 39 different acetogenic bacteria in 25 different media at temperatures between 20 °C and 65 °C. Combined analysis of the autotrophic biomass- specific and volumetric acetate productivities reduced the number of strains studied to 15, of which seven were ultimately identified as strains that may in future be viable systems for the heterologous expression of acetone. These strains were transformed with a variety of plasmids, and subsequent gas chromatographic analyses demonstrated proof of principle for the produc- tion of acetone from a number of different strains under conditions of het- erotrophic cultivation. It is therefore in principle possible to cultivate acetogenic, and even ther- mophilic bacteria, as CO2-consuming acetone producers. However further research is required before the technology is mature enough for use in an industrial production environment. One example of the need for continued research was the realisation that both plasmid stability and plasmid detec- tion in the strains under study were inadequate. University of Ulm The genome of Clostridium aceticum has been fully sequenced and anno- tated. The genome comprises 4.2 megabase pairs with 35 % GC content and contains 3984 genes. During the project, a total of 20 acetone synthesis operons and plasmids were produced that were optimised for acetogenic bacteria. These were in- troduced into the acetogenic bacteria Acetobacterium woodii and Clostridi- um ljungdahlii by vector transformation. A total of 22 different recombinant A. woodii strains and eight different recombinant C. ljungdahlii strains were produced for acetone production. The uncontrolled batch experiments us- ing a gas mixture of hydrogen and carbon dioxide as the energy and carbon source yielded a maximum of 20.6 mM (i.e. 1.2 g/l) of acetone. 65PDF Image | Chemical Processes and Use of CO2
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