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GREEN CHEMISTRY CO2 extraction Limonene Orange Rind

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GREEN CHEMISTRY CO2 extraction Limonene Orange Rind ( green-chemistry-co2-extraction-limonene-orange-rind )

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*Safety Precautions* Figure 1: The phase diagram for carbon dioxide. Special care is required when the tube is in the water bath because the tube could explode or the lid could fly off due to the high pressure created in the centrifuge tube during the extraction. Use only the equipment and methods mentioned here, and do not, under any circumstance, use glass products as a substitution. It is highly recommended to perform this experiment in the hood. PROCEDURE 1. Preparing the Orange rind (or any substance you bring in). Grate only the colored part of the peel of 1/2 of a medium-sized orange with the smallest grating surface of a standard cheese grater or zester into a pre-weighed weighing boat. Make sure you have at least 1.5 g of grated orange rind. 2. Extraction Vessel: Record the mass of a 15 mL centrifuge tube. To extract larger sample sizes we have 50 mL conical vials available. Using a 20 cm piece of copper wire and a piece of 1.5 cm filter paper1, make a solid trap as shown below. Wrap three coils of wire such that the apparatus fits inside the tube with the coils stopping near the beginning of the taper. Use the rest of the wire as a handle projecting up from the coils. All wire should be inside the tube. Cut off any extra wire. Put one piece of filter paper between the coils, making a base to support the solid, and fold up the edges around the wire. Slide the solid trap into tube. Add about 1.5 g of grated orange rind. Do not pack tightly as a tight pack may prevent liquid carbon dioxide from freely passing through. 1 Note: If you are extracting a finely powered spice then it is recommended that you place the spice in an empty tea bag (provided for you) and seal. The tea bag filtration will keep the spice particulates from mixing with your extracted essential oil.

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