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The extraction rate increased when the initial amount of butanol was increased from 1% to 2-3%. The extraction rate of the system for runs with 2 and 3 wt.% were comparable. This may decrease the importance of genetically engineering the organism to survive at 3 wt.% butanol if the extraction rate at 2 wt.% is comparable. Lastly, the models that were adjusted to fit the system’s parameters can be used to predict information about the mass transfer coefficient and extraction rate of the system. The experimental model uses collected data to estimate the initial Kla. The effect of pressure, mass flow rate, and initial concentration on the mass transfer coefficient was analyzed using the experimental model. It was found the initial Kla experiences slight increase with an increase in concentration. However, since the concentration of butanol decreased with time, the overall Kla should not be affected by concentration. Through testing pressures ranging from 1500 PSI to 2000 PSI, it was found the initial Kla was not impacted with a change in pressure. We believe that the range of pressures tested was not significant enough to impact the mass transfer coefficient, but the system could not be run at much higher of a pressure. Increasing the mass flow rate of the supercritical carbon dioxide solvent from 1.26 to 9 mL/min caused the Kla of the system to increase. This result makes sense since an increase in the flow of the solvent through the aqueous butanol solution would increase the amount of surface area and therefore should help improve the mass transfer rate. The theoretical model was created to validate the experimentally determined Kla. When assuming a realistic interfacial surface area, three different theoretical models for Kla were found to be around the experimental Kla value, validating the experimental Kla value. Then the experimental Kla value was used in the same experimental model to predict extraction results at various pressures, initial concentrations, and mass flow rates. When overlaying predicted trends created by the model with experimental data at various conditions, the trends accurately imposed on the data points. Therefore, we conclude that adjusting Tai and Wu’s model from an ethanol extraction system to a butanol extraction system with supercritical carbon dioxide, it can accurately predict extraction results and the mass transfer coefficient of our system. This model can be used to predict extraction results at conditions that have not yet been tested on the system. 60PDF Image | Extraction of Bio-Butanol using Supercritical Carbon Dioxide
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