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Chapter 6: Recommendations and Future Work After completing our project, our team has a few recommendations for operation of the system, the next steps for research, and safety improvements of the system in the future. With regards to the operation of the system, we recommend to run the extraction unit in 30-minute intervals. We found that in the first 30 minutes, 80% of the total amount of butanol in the system was extracted. In 60 minutes, close to 100% of butanol can be extracted, however we believe it is more efficient to obtain 80% of butanol in 30 minutes rather than 100% in double the amount of time. In addition, we have a few recommendations for the next steps of research to reach the end goal of efficiently extracting alcohols with the organism residing in the system. To start off, the system should be tested with all appropriate alcohols besides butanol, produced by B. Megaterium. This will provide predictions for the extraction rates of all potential types of alcohol. After all alcohols are tested in the system, the organism can be introduced in a semi-batch mode. A semi-batch mode is recommended because we believe it would be the most effective set-up when considering economics and safety. Once data is obtained for the semi-batch mode containing organisms, the experimental and theoretical models our team developed can be modified to incorporate any affects the organism may have on extraction results. Lastly, the theoretical model for interfacial area (a) should be further investigated to increase the accuracy of the theoretical model. This can be done by finding a correlation for interfacial area (a) that accurately represents the parameters of our system. To improve the safety of the systems operation we recommend developing a safer method to depressurize the system. The ideal method would take a shorter amount of time and would not run the risk of freezing the line through which the system is depressurized. To add to this, our team recommends that the plastic shields surrounding the system remain on throughout the entirety of every run to protect the researchers. We also recommend a replacement of plastic tubes connecting the collection jars with a material that can withstand higher temperatures. This will allow the use of heating tape on the lines connecting the sample jars. The heating tape would reduce the possibility of the buildup of pressure from the lines freezing over. Lastly, the team recommends the use of double layer 61PDF Image | Extraction of Bio-Butanol using Supercritical Carbon Dioxide
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